Women's second seed Victoria Azarenka and men's sixth seed Jo-Wilfried Tsonga were among those who withdrew.
Organisers rejected Azarenka's claims that the courts were of poor quality, describing them as "high quality".
But Maria Sharapova, who lost to Michelle Larcher De Brito on court two, referred to the surface as "dangerous".
The 2004 champion fell three times and required lengthy treatment on her hip in the second set as she went out 6-3 6-4.
Earlier, Steve Darcis, who beat Rafael Nadal in the first round, pulled out with a shoulder problem, while both Marin Cilic and John Isner retired with knee injuries and a left hamstring problem ended Radek Stepanek's campaign.
Yaroslava Shvedova then also withdrew ahead of her second-round match with eighth seed Petra Kvitova, citing an injury to her right arm.
"There has been a high number of withdrawals at the Championships today and we sympathise with all the players affected," said Wimbledon chief executive Richard Lewis in a statement.
"The withdrawals have occurred for a variety of reasons, but there have been some suggestion that the court surface is to blame. We have no reason to think this is the case."
Two-time semi-finalist Azarenka fell heavily during Monday's 6-1 6-2 first-round win over Maria Joao Koehler of Portugal and required lengthy treatment before continuing with heavy strapping on her knee.
"The court was not in a very good condition that day. My opponent fell twice and I fell badly," said Azarenka.
"I don't know if it's the court or the weather. I can't figure it out.
"There is nothing I've done wrong that caused me to just withdraw from Wimbledon."Lewis defended the court preparation, adding it was to "exactly the same meticulous standard as in previous years and it is well known that grass surfaces tend to be more lush at the start of an event.
"The factual evidence, which is independently checked, is that the courts are almost identical to last year, as dry and firm as they should be, and we expect them to continue to play to their usual high quality."
"Although a number of players have withdrawn injured, only one player has attributed this to slipping over on court."
Tsonga also withdrew with a knee problem while trailing Ernests Gulbis 3-6 6-3 6-3. The number six seed required treatment at the end of the second set but he failed to recover and pulled out at the end of the third.
Darcis, who was scheduled to play Lukasz Kubot of Poland, pulled out before the game could get under way."The injury happened against Rafa in the middle of the first set when I fell down," he said."A few hours after the Nadal match, I start to feel so much pain, I couldn't sleep that night.
"I saw the physio and the doctor yesterday. They did a good job. It's a little bit better today. But no chance I can play."Both Isner and Stepanek required treatment during their second-round matches but also failed to recover enough to continue.
American Isner had played just two games of his match against Adrian Mannarino of France when he was forced out with an injury to his left knee."I always serve and land on my left leg, like I have done 20 million times playing this game, and this is the first time I just felt this sharp pain," he said.
Stepanek soon followed, trailing one set to love and 5-3 to 24th seed Jerzy Janowicz, when he retired with a hamstring problem.Cilic made it five withdrawals when he pulled out ahead of his second-round match with Frenchman Kenny De Schepper.
Title: Victoria Azarenka Wimbledon pulls out
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Rating: 100% based on 99998 ratings. 5 user reviews.
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